What is DNA-helicase? Posted in Genetics The unwinding process of two strands of DNA is catalyzed by enzymes called DNA helicases. The major DNA helicase in E. coli to the product of the dnaB gene. The helicases unwind DNA molecules using energy derived from ATA. Share this:EmailFacebookGoogleRedditLike this:Like Loading... Related posts: what is ø replication? What is oncogene? Radioactive substance in genetic experiments?