1. Make faecal smears as for ZN and fix in methanol.
2. Stain with Auramine-Phenol (Lemperts) for 10-15 min.
3. Rinse thoroughly in tap water.
4. Decolourise in acid alcohol (as for ZN).
5. Rinse thoroughly in tap water.
6. Counterstain with 0.1% potassium permanganate for 30 sec.
7. Rinse thoroughly in tap water, allow to air dry. Do not blot dry, many brands of blotting paper will fluoresce!
Results- Oocysts appear as bright yellow discs against a dark background.