Preparation of Whole Mount
What is whole mount?
Placing a whole organism or specimen on a slide for microscopic examination.
Preparation of whole mount
Single staining with Borax carmine:
- Formalin preserved material should be washed through two or three changes of water till the fixative is washed out completely. In case the material is preserved in alcohol, omit this step.
- After washing, dehydrate the material by transferring it through 30, 50 and 70 parcent alcohol. Leave for 5 minutes in each solution. In case of alcohol preserved tissues transfer the material directly from 70% alcohol to Borax carmine stain.
- Stain in Borax carmine and leave for 10 to 15 minutes depending on the thickness of the material.
- Wash the material, if over stained then remove the excess stain by acid alcohol (1 % HCL in 70% alcohol).
- Wash the material in 90% and then to absolute alcohol and keep for 10 minutes in each.
- Remove alcohol by clearing in xylol for 10 minutes.
- The material is then temporarily or permanently mounted on a slide. For temporary preparation the material is kept on the glycerine placed on a clear grease free slide and is covered with a cover-slip. After placing cover slip slight pressure is applied on the cover-slip so that excess glycerine and air bubbles come out. If still air bubbles remain present under the cover slip, slides should be placed on Hot plate for few minutes to make it air free.
- For permanent mounting the above mentioned procedure is applied. But instead of glycerine Canada Balsam or DPX is used.